Antisense_RNA_and_global_regulation_ques

Antisense_RNA_and_global_regulation_ques - E. coli Trp...

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E. coli Trp Attenuator Assume that the U in the stop codon is replaced by a C due to a mutation. A. What effect might this have on: 1. translation of the leader mRNA? 2. the effectiveness of the attenuator? B. How could you experimentally test: 1. your prediction about the translation of the leader mRNA? What do you need to execute your experiment? 2. the effectiveness of the attenuator? What do you need to test to carry out the appropriate experiment?
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P copA copB repA tap oriR1 CopT RNA CopT + CopB SD SD SD CopT/CopA RNA duplex RepA SD = Shine Dalgarno box tap= leader peptide RepA is functionally related to DnaA 5' 3' 3' 5' 5' 5' 5' 3' 3' 3' What would happen if the DNA that is transcribed into copT is replaced by a completely different sequence? (Consider SD box).
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What might be the effect of a mutation in the hpr gene that results in the replacement of the critical His or Ser, respectively, in Hpr with Ala? Glucose Glucose-6-P PTS-mediated glucose transport HPr - His ~P PE~P Pyruvate EI out in EIIC EIIB ~ P P EIIA
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Antisense_RNA_and_global_regulation_ques - E. coli Trp...

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