Sample_Test_Questions

Sample_Test_Questions - Sample Test Questions from Days...

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Sample Test Questions from Days Gone By A. Multiple Choice 1. Which is NOT a trans -acting factor involved in chromosome replication? A. DnaG B. DnaB C. DnaA D. oriC 2. The activities of DNA Polymerase I include: A. a 5'- 3' exonuclease. B. an RNA polymerizing activity. C. a 3'- 5' DNA polymerizing activity. D. a ligase activity. 3. A 3' - 5' exonuclease "proofreading" or editing activity is: A. found in DNA PolI but not DNA PolIII holoenzyme. B. found in DNA PolIII holoenzyme but not DNA PolI. C. is found only in bacteria with linear chromosomes. D. provided by the e subunit of DNA PolIII (encoded by dnaQ ). 4. Which statement is false? A. AT base pairs have three hydrogen bonds. B. AU base pairs are found in the chromosome while replication is in progress. C. The bacterial chromosome is about 1,000 time longer than the cell measurement. D. Bacterial nucleoids are not surrounded by a membrane. 5. Okazaki fragments are synthesized by: A. primase and DNA Pol I. B. primase and DNA Pol III. C. DNA PolIII and DNA Pol I. D. DnaA and primase.
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6. Which statement is not true with respect to compounds that inhibit replication or affect DNA structure? A. Resistance to the compound trimethoprim can be due to an increase in copy number of the dihydrofolate reductase gene. B. Dideoxynucleotides can be incorporated into DNA but then stop replication. C. Dideoxynucleotides stop replication because they cannot be incorporated into DNA. D. Nalidixic acid and novobiocin kill cells because they bind to DNAgyrase. 7. The Dam enzyme methylates adenines: A. everywhere they occur in DNA. B. on both strands at the sequence 5' GATC 3'. C. on only one strand at the sequence 5' GATC 3'. D. to identify the primer portion of an Okazaki fragment. 8. Which statement is true with respect to a comparison of a fast-growing E. coli culture and a slow-growing E. coli culture, if both are grown at 37C. A. Replication of the chromosomes in the fast-growing culture occurs at a faster rate of polymerization. B. The fast-growing bacteria are much smaller. C. The fast-growing bacteria have a higher DNA content/cell. D. The replication forks move slower in the slow-growing bacteria. 9. Polymerases synthesize nucleic acids in the 5' to 3' direction in: A. DNA, but not RNA, B. RNA, but not DNA, C. all DNA and RNA D. some DNA and RNA 10. The primers in DNA replication are needed to: A. distinguish parental DNA from newly synthesized DNA. B. provide a 3'-OH for DNA polymerase to add a nucleotide. C. keep DNA strands at the replication fork in a single-stranded state. D. unwind the DNA helix.
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11. Which of the following is a process involved in the exchange of genetic material between organisms? A. Transformation B. Transcription C. Transaction D. Translation 12. Which of the following is an enzymatic activity associated with DNA polymerases and functions to reduce the occurrence of spontaneous mutations during DNA replication: A. DNA Pol 5' ÿ 3' exonuclease activity B. DNA Pol 5' ÿ 3' polymerase activity C. DNA Pol 3' ÿ 5' exonuclease activity D. DNA Pol 3' ÿ 5' polymerase activity 13.
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This note was uploaded on 05/01/2010 for the course MMG 431 taught by Professor Bertrand during the Spring '08 term at Michigan State University.

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Sample_Test_Questions - Sample Test Questions from Days...

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