Lab10-cl - Lab 10. Mixed unknowns, Pathogens Before we...

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Lab 10. Mixed unknowns, Pathogens Before we start…. - Turn in the lab report (Lab 8 & Lab 9, before 6:10 pm) - Wipe the bench with disinfectant spray/wash your hands
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This week’s activities You should understand the function of the components in the media and reagents responsible for the end result observed . You need to come back to lab 4 days in a row to check your results and set up the next step in the experiment Come during open lab hours during weekend (10 am -5 pm) The physiological basis of biochemical differential test is the presence or absence of certain enzymes and metabolic pathways in the organism being studied. Based on several biochemical differential tests, you will learn how to identify an organism in a mixture.
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This week’s activities Escherichia coli Klebsiella pneumoniae Serratia marcescens Shigella flexneri Salmonella typhimurium Edwardsiella tarda Providencia alcalifaciens 2. You will conduct a series of cell culture and biochemical tests to identify your three unknown species . Enterococcus faecalis Streptococcus agalactiae Staphylococcus aureus Enterics (7 species) Cocci (3 species) 1. Each of you will sign-out your “unknown” mix (one Gram positive cocci and two Gram negative Enterics) Listed on Lab X-19
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What is pathogen? Pathogen is a biological agent that causes disease or illness to its host. This means you will need to be really careful to protect yourself from your unknown. No chewing gums or candy etc. while you are in the lab. Drink bottles should stay outside of the lab area (in the hall locker). Pathogenic Cocci: Gram positive, have more complex nutritional requirements, a lot of them cause hemolysis on blood cells . Enterics: A group of closely related bacteria commonly found in the intestines of humans, both healthy and diseased. - Gram negative rods, straight or slightly bent - Facultative anaerobes (therefore, catalase positive), non spore forming - Glucose fermenters, - Non-motile or motile with flagella arrangement
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Direct antibiotic sensitivity tests find antibiotic sensitivity patterns. Fluorescent antibody staining rapid direct detection and identification of certain pathogens by anti-body conjugated fluorescent dye, but cannot substitute for pure culture isolation and identification. Isolation and identification of pure cultures of pathogens. Serologic identification using antisera specific for the pathogen or for certain of its toxins. General diagnostic procedures (Lab X-1) Collecting the specimen (e.g. sputum, pus, sediments of urine, and spinal fluid) without contamination. Direct smear and stains will give you clues as to the types of predominant microorganisms. Culture of the specimen for isolation of the pathogen
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This note was uploaded on 05/02/2010 for the course BIO 126L taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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Lab10-cl - Lab 10. Mixed unknowns, Pathogens Before we...

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