SP 2010_-_Replication of Ecoli

SP 2010_-_Replication of Ecoli - o 3’ 5’ exonuclease...

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Replication of E.coli (Prokaryotic) Requirements OriC - the origin of replication DnaA protein - bind to OriC and cause strand separation DNA gyrase - help unwinding DnaB (helicase) - separate the strands and move in the 5’ 3’ SSB (single strand binding protein) - is on the single strand and keep the strand separated Primsome - synthesize the RNA primer with the enzyme DnaG (primase) Poly III - catalyze DNA synthesis o 5’ 3’ polymerase
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Unformatted text preview: o 3’ 5’ exonuclease • Clamp loader ( γ ) - open the β clamp to load it onto the DNA template • Sliding clamp (β)- increase Poly III processivity • Poly I- remove RNA primer (5’ 3’ exonuclease) and replace with DNA (5’ 3’ polymerase activity) • DNA ligase- seal the gaps between the Okazaki fragments • Tus protein- bind to Ter site, which prevents DnaB helicase and blocks directional valve...
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This note was uploaded on 05/03/2010 for the course CHEM Chem 114C taught by Professor Hoffman during the Spring '07 term at UCSD.

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SP 2010_-_Replication of Ecoli - o 3’ 5’ exonuclease...

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