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Unformatted text preview: Introduction: Gel electrophoresis is use to separate macromolecules such as DNA molecules and enzymes (proteins) on based on their size, shape and electric charge. In this method, enzyme solution is run under electrical PJQEDPWCW current and after that the enzyme solution is treated with selective histoehemical stain. The stain helps to visualize . “ flier; 'WL) the enzymes’ travelling band patterns under visible light. The enzymes of interest are isoenzymes, which differ ‘ / slightly in the amino acid composition, but perform the same reaction on the same substrate. 1 will use Gel {fr/£251 ~5- electrophoresis and isoenzymes of different-strings of Drosophila fly to support my hypothesis, which ism- ,_, 3.33:6 morphological differences, three different strings of Drosophila flies will have hidden variation in their enzyme , . . EASrCJD E353 WWfi/sqfogtdfli DJFI-(WLLE) :1:— f—Qx/fl» 79:? STf'fegifh-Ij E‘OChemmanyf “owl! Hat-V31 gum-Hr; Dr) erfi 53> {.3 Jimmie a c 405 11?) Zr; Men" (3+2: :5. . , w Materials and Method: In order to test the predictio I performed an experiment according to the Introductory \f}, 13: fl Genetic Lab Manual (Biology department, zoom/4:1 three different kinds of flies in this experiment: 1. (+) wild A, mfg/t K 0-; (a type flies (D. melanogesrer), 2. MRC CnyM D/Sb flies, 3. Virilis flies (D. virilr' As stains, I used Alcohol (A‘U‘j’K Dehydrogenase (ADH), Malate Dehydrogenase (MDH) and Aldehyde Oxidase (AOL/ 2“ 344:7 Result: The enzymes passed from the positive terminal towards the positive terminal on the ADH stain. The only band for wild type enzyme'was the slowest and therefore, the position of the band was the closest from the starting I % __.———e»Do..J 34:; #15 S é point among the three enzymes’ hand (Figure 1).."l'hus, I assumed the ' type BMW um Ii}. (US-SN (Griffiths et al, 2008). The marker stain enzyme had three ban ' attem in relatively close distance from wild type I 7’ enzyme (Figure 1). Therefore, I considered themzymuohehetemzygous-(Griffiths et a1. 2008). The D. vii-His enzyme had one banding pattern and the position of the hand was the farthest out of all three enzymes’ bands (Figure 1) andI assumed that the enzyme to he homozygous (Griffiths et at, 2008). Again, on the MDH stain, the enzymes traveled from the negative terminal towards the positive terminal. However, in this stain the enzyme showed a continuous banding pattern. The banding pattem position of D. virilis was the was unable to determine their genot . farthest among three enzymes, then Win and then followed by wild type enzyme (Figurel). Therefore, I And, on the A0 stain, the enzymes moved from the positive terminal towards the negative terminal. The banding pattern of the D. virilis enzyme appeared closest to the starting point, then the marker enzyme and then the wild type enzyme We farthest position (Figure l). I considered all three enzymes to be homozygous (Griffiths er of, 2003 ...
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