10-151_lab_manual - BIOLOGY 151 LABORATORY MANUAL...

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BIOLOGY 151 LABORATORY MANUAL INVESTIGATIONS IN GENETICS AND EVOLUTION Spring 2010 KELLAR AUTUMN, GARY REINESS, STEVEN SEAVEY and GRETA BINFORD With contributions by Wendy McLennan
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2 TABLE OF CONTENTS Page Semester schedule 3-4 Introduction 5-10 Working with Drosophila 11-17 Investigation #1 -- Inheritance of white eye phenotype 18-20 Investigation #2 -- Determining gene location 21-28 Summary of Investigation #1 & #2 procedures 29 Mitosis and Meiosis 30-31 Introduction to Statistical Analysis of Data 32-35 Timing of development of Drosophila , egg to adult 36 How to write and submit scientific papers for this class 37-40
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3 Schedule Week of Mon Lab Activity Jan 18 Introduction: Getting organized. Intro to using a lab notebook. Jan 25 Form lab teams. Working with Drosophila melanogaster: w ild type & mutants. Intro to Investigation #1 and Investigation #2. Before coming to lab, read lab manual pp. 5-17, and p. 29. Read Morgan 1910. http://www.esp.org/foundations/genetics/classical/thm-10a.pdf .. Feb 1 Investigation #1: Before coming to lab, read lab manual pp. 18-20. Count & phenotype F1. Establish F1* F1 cross Investigation #2: Before coming to lab, read lab manual pp 21-23. Before next week's lab, isolate unmated females for backcrosses in Investigation #2. Subculture P linkage flies. Lab Quiz #1. Feb 8 Meiosis exercise: Shuffling chromosomes Before coming to lab, read lab manual pp. 30-31, and read Chapter 2 in Klug. Investigation #1: Morgue adults. Inv. #2: Before coming to lab, read lab manual pp. 24-28. Establish crosses. Predictions for F1 gen due (oral exam by individual). Feb 15 Investigation #1: Count and phenotype F2. Chi-square analysis of data . Investigation #2: Morgue adults. Before next week's lab, isolate unmated females for backcrosses in Investigation #2. Segregation ratios in corn and M&M’s; application of statistical inference (hypothesis tests). Before coming to lab, read lab manual pp. 32-35 Feb 22 Lab Quiz #2 Investigation #2: Count and phenotype F1s. Establish backcross of P females and selected F1 males. Predictions for Backcross generation due (oral exam by individual). Mar 1 Investigation #2: Morgue adults.
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Mar 8 Inv. #2: Count and phenotype backcross generation. Data analysis. Mar 15 Investigation #2: Before coming to lab read lab manual pp.37-40. Assistance with paper. Papers submitted (Due Friday, March 19) Mar 22 Spring Break Mar 29 Investigation #2: Papers returned for revisions. Authors of rejected papers must meet with lab instructor Intro to Symposium Apr 5 Continue Symposium research. Final Paper 2 due in your lab instructor’s Biology mailbox on Fri 4/9 by 4:00 pm sharp. Apr 12
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This note was uploaded on 05/03/2010 for the course BIO 151 taught by Professor Autumn during the Spring '10 term at Lewis and Clark Community College.

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10-151_lab_manual - BIOLOGY 151 LABORATORY MANUAL...

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