10-151_lab_manual - BIOLOGY 151 LABORATORY MANUAL...

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BIOLOGY 151 LABORATORY MANUAL INVESTIGATIONS IN GENETICS AND EVOLUTION Spring 2010 KELLAR AUTUMN, GARY REINESS, STEVEN SEAVEY and GRETA BINFORD With contributions by Wendy McLennan
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2 TABLE OF CONTENTS Page Semester schedule 3-4 Introduction 5-10 Working with Drosophila 11-17 Investigation #1 -- Inheritance of white eye phenotype 18-20 Investigation #2 -- Determining gene location 21-28 Summary of Investigation #1 & #2 procedures 29 Mitosis and Meiosis 30-31 Introduction to Statistical Analysis of Data 32-35 Timing of development of Drosophila , egg to adult 36 How to write and submit scientific papers for this class 37-40
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3 Schedule Week of Mon Lab Activity Jan 18 Introduction: Getting organized. Intro to using a lab notebook. Jan 25 Form lab teams. Working with Drosophila melanogaster: w ild type & mutants. Intro to Investigation #1 and Investigation #2. Before coming to lab, read lab manual pp. 5-17, and p. 29. Read Morgan 1910. http://www.esp.org/foundations/genetics/classical/thm-10a.pdf .. Feb 1 Investigation #1: Before coming to lab, read lab manual pp. 18-20. Predictions for F1 & F2 generations due (oral exam by group). Count & phenotype F1. Establish F1* F1 cross Investigation #2: Before coming to lab, read lab manual pp 21-23. Before next week's lab, isolate unmated females for backcrosses in Investigation #2. Subculture P linkage flies. Lab Quiz #1. Feb 8 Meiosis exercise: Shuffling chromosomes Before coming to lab, read lab manual pp. 30-31, and read Chapter 2 in Klug. Investigation #1: Morgue adults. Inv. #2: Before coming to lab, read lab manual pp. 24-28. Establish crosses. Predictions for F1 gen due (oral exam by individual). Feb 15 Investigation #1: Count and phenotype F2. Chi-square analysis of data . Investigation #2: Morgue adults. Before next week's lab, isolate unmated females for backcrosses in Investigation #2. Segregation ratios in corn and M&M’s; application of statistical inference (hypothesis tests). Before coming to lab, read lab manual pp. 32-35 Feb 22 Lab Quiz #2 Investigation #2: Count and phenotype F1s. Establish backcross of P females and selected F1 males. Predictions for Backcross generation due (oral exam by individual). Mar 1 Investigation #2: Morgue adults.
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4 Mar 8 Inv. #2: Count and phenotype backcross generation. Data analysis. Mar 15 Investigation #2: Before coming to lab read lab manual pp.37-40. Assistance with paper. Papers submitted (Due Friday, March 19) Mar 22 Spring Break Mar 29 Investigation #2: Papers returned for revisions. Authors of rejected papers must meet with lab instructor Intro to Symposium Apr 5 Continue Symposium research. Final Paper 2 due in your lab instructor’s Biology mailbox on Fri 4/9 by 4:00 pm sharp. Apr 12 Symposium prep continued. Apr 19 Symposium Presentations. Apr 26 No lab; use time to study for final exam!
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5 Introduction Learning Objectives This lab is designed to introduce you to the methods that are used to investigate genetic mechanisms of inheritance. We will do this by immersing you in investigations that will teach you scientific methods of inquiry in these fields. Specifically, we have designed this lab to be an opportunity for you to:
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