BIO212RespPhysiol2Protocol

BIO212RespPhysiol2Protocol - BIO 212 ANATOMY AND PHYSIOLOGY...

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BIO 212 ANATOMY AND PHYSIOLOGY II RESPIRATORY FUNCTION TESTING: THE MEASUREMENT OF STATIC LUNG VOLUMES AND CAPACITIES IN HUMANS
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A. INTRODUCTION A simple method for studying lung function is to record the different volumes of air which can be moved voluntarily by a subject in response to varying inspiratory and expiratory efforts. The process of recording the different volumes is called spirometry and the record generated is called a spirogram . We will generate a “spirogram” indirectly by measuring air flow rates using a device called a pneumotachograph and then integrating this record to generate a volume trace. The various subdivisions of lung air (i.e. volumes and capacities) are described in Figure 1 below. Figure 1. Diagram illustrating respiratory excursions During normal breathing and during maximal inspiratory and expiratory efforts B. OBJECTIVE Using one subject, you will generate data which will allow you to determine static lung volumes and capacities. 2
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C. CALIBRATION PROTOCOL (a) Be sure that the airflow (pneumotachograph) transducer ( SS11LA ) is connected to the CH 1 port on the MP30 unit. (b) Using the left-hand side of the mouse button, double click on the Biopac Student Labs icon. (c) Select (double click on) “ L12-Pulmonary Function 1”. (d) Assign a filename as follows: RP ( Lab section )( Group # )(e.g. “ RPB3 ”) (e) Click on “ OK” . (f) Prepare to calibrate the airflow transducer by inserting the filter/calibration syringe assembly into the transducer port (marked “Inlet” ) as shown below in Figure 2 . Never hold onto the airflow transducer handle when using the calibration syringe or the syringe tip may break . Figure 2. Calibration assembly (g) The airflow transducer is sensitive to gravity so it needs to be held vertically throughout both calibration and recording ( Figure 3 below). Figure 3. Holding the calibration unit 3
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C. CALIBRATION PROTOCOL (continued) (h) Pull the plunger all the way out and, then, click on Calibrate (upper left- hand corner of the screen). The first calibration stage will run for 8 seconds and end with an alert box. Do not touch the plunger during this stage because any pressure or movement will cause inaccurate results . (i) Carefully read the directions in the journal (beneath the screen recording area) so that you fully understand the second stage calibration procedure.
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BIO212RespPhysiol2Protocol - BIO 212 ANATOMY AND PHYSIOLOGY...

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