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d1s80 - GTCTT GTTGG AGATG CACGT GCCCC TTGC 5’ primer(16...

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PCR of human D1S80 Locus • D1S80 – short tandem repeat (STR) region on chromosome 1 6 bp repeat unit 16 bp repeat unit – GAGGA CCACC (G/A)G(C/A)AA G • 27 known alleles in human population – 14 - 41 repeats – 224 – 656 bp • 729 possible genotypes • Individual can be either homozygous (20%) or eterozygous (80%) heterozygous (80%)
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D1S80 PCR primers 3’-primer AAAC TGGCC TCCAA ACACT GCCCG CG GAAAC TGGCC TCCAA ACACT GCCCG CCG 5’-primer TCTT GTTGG AGATG CACGT GCCCC TGC
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Unformatted text preview: GTCTT GTTGG AGATG CACGT GCCCC TTGC 5’ primer (16 bp) n 3’ primer n = 14 to 41 D1S80 alleles have ifferent frequencies different frequencies in various human populations Budowle et al. 1997 http://www.astm.org/DIGITAL_LIBRARY/ OURNALS/FORENSIC/PAGES/JFS14219J htm JOURNALS/FORENSIC/PAGES/JFS14219J.htm Budowle et al. 1995 http://www.astm.org/JOURNALS/FORENSIC/ PAGES/2466.htm http://www.astm.org/JOURNALS/ FORENSIC/ PAGES/JFS45101X18X.htm Budowle et al. 2000...
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d1s80 - GTCTT GTTGG AGATG CACGT GCCCC TTGC 5’ primer(16...

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