PreLab_IronCereal

PreLab_IronCereal - C125 Ex rimntal Che istry 1 pe e m Iron...

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Iron in Total Cereal C125 Prelab C125 Exp er im en tal Che m i st ry 1
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Experimental Purpose Investigate what is meant by: Beer-Lambert Law Absorbance-Transmittance relationship Determine : Iron concentration by using absorption spectroscopy
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Important Terms for This Experiment Spectrophotometer Absorbance Transmittance Beer-Lambert Law Molar absorptivity Path length
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Spectrophotometer Measures the wavelength at which a sample absorbs Quantifies how much a sample absorbs at any given wavelength Measures the light intensity that is emitted and the % transmittance Light hitting an object may be reflected, transmitted, and/or absorbed Amount of light absorbed used to determine sample concentration Use a wavelength of 510 nm. What color does that correspond to?
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Spectrophotometer 100% control knob Wavelength knob 0% adjust/power knob Cell holder Wavelength display Transmittance display Wavelength filter
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Spectrophotometer Light Source emits light at a specific wavelength with intensity I o Sample absorbs, reflects, or transmits light Light Detector measures transmitted light to find intensity I
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Spectrophotometer A wavelength of 510 nm corresponds to the color blue-green This wavelength is reflected by things that appear blue-green This wavelength is absorbed by things that appear all other colors The mix of all other colors will appear red- orange, which is complementary color of blue- green. Why is this important for our experiment?
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Color Wheel 400nm 450nm 475nm 500nm 525nm 550nm 575nm 600nm 625nm 650nm 700nm 800nm
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Definition of Transmittance The amount of light that passes through the sample % Transmittance = amount of light passed through the sample to the detector divided by the amount of light emitted by the light source x 100% %T = I/I o x 100%
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