Ex1Microscopy09

Ex1Microscopy09 - K101 LAB EXERCISE 1 MICROSCOPY AND CELL...

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K101 LAB EXERCISE 1 MICROSCOPY AND CELL STRUCTURE The world is full of magic things waiting patiently for our senses to sharpen.” --John Keats” This exercise is designed to guide you in learning the parts of a light microscope and to teach you the uses as well as the limitations of this very important biological instrument. You will have a chance to practice using both the compound light microscope and the dissecting microscope. While working through this exercise refer to Figs. 1 and 2 to identify the various parts of the microscopes you are using. A. Care of the Microscope Because microscopes are carefully made scientific instruments containing many specially tooled parts (such as lenses) they are relatively expensive. A student-grade light microscope costs about $2,000 - $5,000. A research-grade microscope in the laboratory costs from $20,000 to as much as $150,000! Thus, great care must be taken in handling your microscope. 1. To carry a microscope, place the palm of your hand beneath the base of the microscope while grasping the arm of the microscope with the other hand. Carry the microscope in an upright position so various parts do not falloff. 2. To clean microscope lenses use only lens paper or cotton swabs. Kimwipes or paper towels contain wood fibers that are abrasive and therefore undesirable for cleaning the lens surface. Use either water or 95% ethyl alcohol to clean lenses of student microscopes. Lens paper squares in covered dishes, and dropper bottles of distilled water and alcohol may be found in the drawers under your laboratory bench. 3. Do not force any adjustments on your microscope. Ask your Teaching Assistant (TA) for assistance if you cannot adjust your microscope satisfactorily. If your microscope does not work properly check first with your TA before putting the defective microscope on the front bench (where it will be tuned up if needed). B. Anatomy of The Compound Microscope : Purpose of this activity: Locate all of the parts mentioned below on your microscope and locate them on the attached diagram. The compound microscope is so-called because it has two sets of lenses, the lens in the eyepiece or ocular and the lens in the objective. Both lenses contribute to the magnification (the amount an image is enlarged) and resolution (the ability to produce a sharp image between of two points such that both points can still be distinguished from each other). 1. Obtain a compound microscope and locate the eyepiece or ocular. Our microscopes have two eyepieces and are called binocular microscopes. If you wear glasses, you may want to see what works best for you – leaving them on or removing them to look through the microscope. The magnification provided by the eyepiece (ocular)
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This note was uploaded on 05/05/2010 for the course BIO 101 taught by Professor Vaughan during the Spring '10 term at IUPUI.

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Ex1Microscopy09 - K101 LAB EXERCISE 1 MICROSCOPY AND CELL...

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