Ex3MacromoleculesFall09-1

Ex3MacromoleculesFall09-1 - K101 LAB EXERCISE 3...

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K101 LAB EXERCISE 3 MACROMOLECULES IN THE DIET: CARBOHYDRATES, LIPIDS, AND PROTEINS NOTE : Please bring in a small amount (1 tsp. or less) of a solid food from home! See Part 6! INTRODUCTION All life is largely composed of compounds made from carbon, hydrogen, oxygen and nitrogen. These atoms can be arranged by cells to make sugars, fatty acids, amino acids and nucleotides - building blocks that form more complicated macromolecule like carbohydrates, lipids, proteins, and nucleic acids. Of these 4 major groups of macromolecules, the first three not only provide that basic ‘building materials’ from which our cells and tissues are constructed, but also provide us with cellular energy (measured in kilocalories) via the covalent bonds between the atoms. (The last group, nucleic acids, make up DNA and RNA. Although we typically consume the DNA and RNA of other organisms, we do not derive a significant nutritive value from these macromolecules, but these polymers can be broken down to their constituent parts and re-used by our cells). During today’s lab, you will learn to do simple assays , or tests, for common macromolecules like sugars, lipids, and proteins, and then use your knowledge of these assays to determine the types of macromolecules commonly found in the foods we eat. During the last two parts of the lab, you will do an unknown determination of a common food substance, and you will also devise a way to test a solid food (from the lab or from a sample you bring in) for the presence of common macromolecules. Please read through this handout carefully before lab . There are many things to do, and it is to your benefit to be as organized as possible so that your time in the lab is spent doing the work to be done rather that trying to figure out what needs to be done by reading through the lab in class! Good organizational skills and teamwork between you and your lab partner (and between the other groups at your bench) will make the procedures go smoothly. You will also see the importance of labeling tubes clearly and making accurate measurements. (These are also important skills for your future as a scientist or medical professional). If you have any questions before proceeding, just ask your TA. Most of the substances we will use today pose no danger in the lab, but continue to follow lab procedures for safety and disposal of waste. CLEANUP Note: After completing all your experiments, please make sure to clean up your workspace. Place tubes for each test BACK IN ORDER in the correct test tube rack. Discard used tips and microfuge tubes. Place pipettemen back in their stands. Wash out your spot plate and dry it upside down on the tray on your bench. Do not throw away any screw-capped tubes – let your TA know if solutions need to be replaced. THANKS for your cooperation ! PART 1:
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This note was uploaded on 05/05/2010 for the course BIO 101 taught by Professor Vaughan during the Spring '10 term at IUPUI.

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Ex3MacromoleculesFall09-1 - K101 LAB EXERCISE 3...

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