pGLO lab: Genetic transformation of E. coli bacteria Purpose: To show that bacteria can pick up pieces of DNA from the environment (or from other cells as shown below), and gain some useful traits in the process. We will also demonstrate how genes are regulated (turned on or off only when certain signals are present, like sugars). Bacteria have a single, large circular chromosome, but most also contain extra DNA plasmids : small, mobile circles of DNA that can be easily swapped between cells through a sex pilus . The bacteria that picks up new genes from other bacteria or from the environment is said to be transformed. Sometimes, useful new genes like antibiotic resistance are passed from bacteria to bacteria this way! ( Useful for the bacteria, that is !!) Sex pilus 1 μ m Would you like a DNA plasmid ? I can copy one of mine for you and we can BOTH have one! What is p GLO ? A recombinant (human made) bacterial p lasmid = 4 useful features: 1. ori = the bacterial ori gin of DNA replication (so the plasmid can reproduce)
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This note was uploaded on 05/05/2010 for the course BIO 101 taught by Professor Vaughan during the Spring '10 term at IUPUI.