3385_Ch13 - Chapter 13 Integrin Signaling in Pericellular...

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13 © 1999 by CRC Press LLC Chapter Integrin Signaling in Pericellular Matrix Assembly Chuanyue Wu Contents I. Introduction A. Interactions Between Fibronectin and the Cell Surface Integrins are Required for the Initiation of Fibronecting Matrix Assembly B. Specific Fibronectin-Binding Integrins Mediate Fibronectin Matrix Assembly C. Integrin Cytoplasmic Domains Regulate Fibronectin Matrix Assembly D. Regulation of Fibronectin Matrix Assembly by Intracellular Protein Kinases E. Regulation of Fibronectin Matrix Assembly by Small GTPases II. Analysis of Fibronectin Matrix Assembly by Fluorescence Microscopy A. Overview B. Protocols C. Technical Comments D. Materials III. Biochemical Analyses of Fibronectin Matrix Assembly A. Overview B. Protocols C. Technical Comments D. Materials IV. Conclusion Remarks Acknowledgments References
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© 1999 by CRC Press LLC I. Introduction The interactions of cells with pericellular fibronectin matrix play critical roles in cell adhesion, migration, growth, survival, differentiation, and gene expression. At least two components contribute specificity to this important recognition sys- tem. Responsive cells must express suitable receptors recognizing pericellular fibronectin matrix. In addition, the assembly of pericellular fibronectin matrix must be properly controlled. Our understanding of the molecular mechanism by which cells control pericellular fibronectin matrix assembly, although still incom- plete, has advanced significantly over the last several years. This was achieved through a combination of biochemical, cell biological, and genetic experimental approaches. In this chapter, I will first discuss, briefly, some of the recent findings on the signaling mechanisms involved in the cellular control of fibronectin matrix assembly, primarily focusing on the roles of integrin signaling. For detailed dis- cussion of fibronectin matrix assembly, readers are referred to several excellent review articles that have been previously published. 1-5 Next, I will describe two basic experimental methods for analyzing fibronectin matrix assembly, and discuss some of the major technical considerations in designing experiments aimed at evaluating cellular activity of fibronectin matrix assembly. Due to space limitation, many other useful methods could not be included; readers are encouraged to consult the original publications listed in the reference sections of this chapter and other review articles. 1-5 Fibronectins are modular, multidomain glycoproteins of approximately 500,000 daltons consisting of repeating homologies of three types. 2,6,7 During organogenesis, fibronectins are major constituents of the extracellular matrix. Inactivation of the mouse fibronectin gene causes early embryonic lethality, 8 demonstrating that fibronectin matrix is indispensable for vertebrate embryogen- esis. In adult mammals, most fibronectin is synthesized by the liver and exists primarily as a soluble plasma protein present at high concentration (about
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This note was uploaded on 05/06/2010 for the course MECH. 28197 taught by Professor Dr.shafii during the Spring '10 term at Sharif University of Technology.

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3385_Ch13 - Chapter 13 Integrin Signaling in Pericellular...

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