Chapter 29 - CHAPTER 29 Supercritical Fluid Chromatography...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
CHAPTER 29 Supercritical Fluid Chromatography The mobile phase is a supercritical fluid (a fluid above its critical T and critical pressure) Supercritical fluid properties (density, viscosity, and refractive index) vary with T & P
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Supercritical Fluids At temperatures and pressures above its critical temperature and pressure ( critical point ), a substance is called a supercritical fluid . The critical temperature is the temperature above which a distinct liquid phase cannot exist. The vapor pressure at its critical temperature is its critical pressure . Where supercritical fluids exist: The forces from the kinetic energy of the molecules exceeds the forces from condensing influence of the intermolecular forces, so no distinct liquid phase exists
Background image of page 2
SFC Mobile Phases Mobile phases should have critical parameters that are easily reached using chromatographic pumps and ovens common to currently used instrumentation. Advantages of supercritical fluids over carrier gasses and liquid mobile phases are in its solubility properties, physical properties, and detector compatibility.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
SFC Separations SFC is a hybrid of gas and liquid chromatography that combines some of the best features of each As in HPLC, variation of the mobile phase composition affects separation In SFC, mobile phase affinity for the analyte is
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/06/2010 for the course CHEM 4414 at Arkansas Tech.

Page1 / 26

Chapter 29 - CHAPTER 29 Supercritical Fluid Chromatography...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online