Chapter 32 - Chapter 32 HIGH-PERRORMANCE LIQUID...

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Unformatted text preview: Chapter 32 HIGH-PERRORMANCE LIQUID CHROMATOGRAPHY High-performance liquid chromatography (HPLC) is the most versatile and widely used type of elution chromatography. In liquid chromatography, the mobile phase is a liquid solvent containing the sample as a mixture of solutes. There are several types of high- performance liquid chromatography, depending on the nature of the stationary phase. They include (1) partition , or liquid-liquid , chromatography ; (2) adsorption , or liquid-solid , chromatography ; (3) ion-exchange , or ion , chromatography ; (4) size-exclusion chromatography ; and (5) affinity chromatography . Instruments for High-Performance Liquid Chromatography Pumping pressure of several hundred atmospheres are required to achieve reasonable flow rates with packing in the 3- to 10- μ m size range, which are common in modern liquid chromatography. As a consequence of these high pressures, the equipment for high-performance liquid chromatography tends to be considerably more elaborate and expensive than that encountered in other types of chromatography. Mobile-Phase Reservoirs A modern HPLC apparatus is equipped with one or more glass or stainless steel reservoirs, each of...
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This note was uploaded on 05/09/2010 for the course CHEM 3245 taught by Professor Anwara.bhuiyan during the Spring '10 term at Arkansas Tech.

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Chapter 32 - Chapter 32 HIGH-PERRORMANCE LIQUID...

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