{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Chapter 32 - Chapter 32 HIGH-PERRORMANCE LIQUID...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 32 HIGH-PERRORMANCE LIQUID CHROMATOGRAPHY High-performance liquid chromatography (HPLC) is the most versatile and widely used type of elution chromatography. In liquid chromatography, the mobile phase is a liquid solvent containing the sample as a mixture of solutes. There are several types of high- performance liquid chromatography, depending on the nature of the stationary phase. They include (1) partition , or liquid-liquid , chromatography ; (2) adsorption , or liquid-solid , chromatography ; (3) ion-exchange , or ion , chromatography ; (4) size-exclusion chromatography ; and (5) affinity chromatography .
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 4
Instruments for High-Performance Liquid Chromatography Pumping pressure of several hundred atmospheres are required to achieve reasonable flow rates with packing in the 3- to 10- μ m size range, which are common in modern liquid chromatography. As a consequence of these high pressures, the equipment for high-performance liquid chromatography tends to be considerably more elaborate and expensive than that encountered in other types of chromatography.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Mobile-Phase Reservoirs A modern HPLC apparatus is equipped with one
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}