Exercise 2 Analysis - Exercise 2: Analysis 1. a. Microscope...

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Exercise 2: Analysis 1. a. Microscope has a built it illuminated source. b. It is binocular. c. The field diaphragm controls the amount of light that enters the condenser and the rest of the microscope. d. The aperture diaphragm acts essentially as a control for resolution and contrast in optical microscopy. By varying the size of the diaphragm opening, the angles of light that strike the specimen will decrease. Hence, the scattering contrast in the specimen will increase. e. As magnification is increased the field of view decreases. Uses low magnification at first allows you facilitate in finding specimens, then once you see them you can increases magnification to examine the specimens further. f. By increasing the magnification of the microscope you are also increasing the depth of focus. g. To clean the surface of optical microscopes you use lens paper, since it enable to not damage the lenses. 2. a. Magnification, Contrast and Resolution are the three major issues of light microscopy. The magnification of a microscope is limited by the phenomenon of diffraction, which is the bending of light around small obstacles and the spreading out of ways past small openings. The problem in contrast is that many microscopic objects are transparent and they don’t create enough contrast to see them. Also Even if we magnify the image of an object such that its different parts fall on different detectors on the retina, we will see nothing unless those parts differ in intensity. Resolution, along with magnification is limited by the wavelength of the light. b. Setting the Kohler 1. First you must constrict the field diaphragm. 2. Turn the condenser height adjustment knob until the leaflets of the field diaphragm are in focus. 3. Turn the condenser centering screws until the leaflets of the field diaphragm are centered in the field. 4. You then adjust the height to make the ring around the border of the view become purple. c. Proper condenser alignment is necessary to focus the microscope on the specimen. Also you make the specimen plane and the plane of the field 1
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Exercise 2: Analysis diaphragm conjugate so that there are three conjugate planes in the microscope, the specimen plane, the intermediate plane and the plane of the field diaphragm. 3.
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This note was uploaded on 05/06/2010 for the course BIO 206L taught by Professor Unknown during the Spring '08 term at University of Texas at Austin.

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Exercise 2 Analysis - Exercise 2: Analysis 1. a. Microscope...

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