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Exercise 3 Analysis - 1 The objectives that require oil...

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1. The objectives that require oil immersion is the 100X objective because it helps to reduced the number of mediums that the light has to pass through thus helps to reduce the amount of reduce the refraction of light that it might have cause without using the oil. The proper way to use the oil immersion technique is to first have the microscope on the 40X objective. Then turn the nose piece so that the 40X and 100X objective is in between the specimen. At this point you place a drop of the oil on the cover slip where the light is illuminating the slip. If you adjust the focal plane you will throw everything out of alignment and will need to refocus the specimen again, since it is highly magnified moving the settings will lose focus. When done looking at the specimen with the 100X magnification you reverse the process. First you rotate the nosepiece out of the 100X objective and clean it using lens paper. Then remove the slide, once you remove you must clean the slide so that there is no oil on the slide and then you can go back to viewing the specimen under the 40X objective. 2. At first glance of the human blood smear slide it is very apparent the amount of red blood cells compared to the amount of white blood cells. Sometimes it was hard to spot the white blood cells. After further examination it is possible to see the red blood cell is pretty plane and smaller than the white blood cell and the white blood cell contains some purple like structures inside of the cell, which are the nucleus. Calculating the volume of the cells using the formula of a sphere is as follows. V = Radius of RBC = 3 V= = 113.04 Radius of WBC = 7 V = = 1436.02 Calculating the surface area of the cells is as follows. 1
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Radius of RBC = 3 V= = 113.04 Radius of WBC = 7 V = = 615.44 The white blood cell is the larger of the two cells that were examined by about 12 times as large as the RBC. The one that is more prevalent in the blood is the RBC, since it plays a greater role in the circulatory system. The ratio by looking under the microscope is about several hundred WBC to one RBC. 3. The simple explanation of Phase Contrast is that small phase shifts of the light waves are passing through a transparent specimen and are converted into amplitude or contrast in the image. Especially since light is traveling through different mediums as it goes through the cell it is easier to see the effects through this imaging. By this, it is able to solve one of the main problems with light microscopy, which is the contrast, and with phase contrast no staining is required.
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