signal transduction practice problems key

signal transduction practice problems key - 2 What aspect...

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Signal Transduction Practice Problems First try these problems from Chapter 14 of your book (Berg et al., Biochemistry 6 th edition ), pages 405-407, Problems 1, 2, 4, 5, 6, 9, and 10. Then try these: 1. Explain how amino acid substitutions in the R or C subunit of cAMP-dependent protein kinase (PKA) could lead to a PKA enzyme that is always active? Amino acid substitutions that render the R subunit unable to bind the C subunit. In this case C could not inhibit R and PKA would be constitutively active. How could amino acid substitutions lead to a PKA enzyme that cannot be activated? An amino acid substitution in the cAMP biding domain that prevents cAMP binding would leave the C and R subunits bound to one another and PKA would remain in the
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Unformatted text preview: 2. What aspect of the insulin response would be affected by permanently inactivating ras GTPase activity? Why? If ras cannot hydrolyze GTP it will be constitutively active, leaving the gene expression aspects of the insulin response permanently turned on. 3. Non-hydrolyzable analogs of GTP (see example below), are often used to study G proteins in the laboratory. How would one of these analogs affect epinephrine signaling? cAMP production by adenylate cyclase? A non-hydrolyzable analog of GTP would leave the G α s in the “on” state. As a result, adenylate cyclase would continue to make cAMP and the epinephrine response would be prolonged....
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