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Unformatted text preview: BIOC 460 Exam 3 - Study Guide
NOTE: A SIMPLE SCIENTIFIC calculator will be required for Exam 3, so be sure to bring one. NO PROGRAMMABLE OR GRAPHING CALCULATORS, AND NO SHARING OF CALCULATORS, WILL BE PERMITTED. 1. The exam covers Lecture 26 (Pyruvate Dehydrogenase) through Lecture 37 (Amino acid metabolism). 2. Concentrate on the material covered in lecture. Use the learning objectives given in the lecture notes to focus on important principles, with any additional notes you added in class. Use the textbook to enhance your understanding of the concepts covered in class or in the posted notes reading another explanation will often help you understand a concept better, with more overall context. 3. Review/work practice problems (including the problems assigned from the end of the chapter), problems worked in class, and quizzes (all posted on D2L). With the exception of the D2L quiz, these are the type of questions you should expect on the exam. See if you can answer questions without looking at the answers. 4. Review old exams and quizzes to see the topics emphasized. Remember, we will not be asking multiple choice or true and false. The topics on this exam were spread over a number of different exams last year. Review the following: Spring 2009 Exam Three Problems: 1,7,9,10,11,12, and 13 Spring 2010 Quiz Four Problems: 1,2, and 5 Spring 2010 Exam Four Problems: All 5. Be able to answer all questions posed on the lecture slides. 6. Just as in the previous exams, you may be asked to apply your understanding of the material to a new protein or hypothetical scenario. I strongly recommend studying in a group and discussing scenarios along the lines of "A newly discovered protein works by a mechanism similar to protein X (that we studying in class), what would happen if.... Or "What would be the effect of a mutation in Protein Y ...." The practice problems have several good examples of this type of question. 7. I gave many examples of how your new understanding of biochemistry/metabolism can be used to explain things we encounter every day (diseases, diets, artificial sweeteners, drugs therapies, herbicides, desert plants and animals, etc). Being able to explain these things on a biochemical level is a great test of your understanding of some basic concepts of biochemistry and metabolism! 8. When studying metabolic pathways focus on: Steps that require ATP, GTP, or UTP/ steps that generate ATP or GTP Steps that require coenzymes, Regulated steps (both allosteric and hormonal regulation) The redox steps (be able to identify the electron donor and the electron acceptor), Metabolic diseases that arise from defects in the pathway Drugs or poisons that alter the function of the pathway. Tissue specificity when I discussed it. In other words, if I highlighted how a particular pathway is used in a particular tissue (e.g. pentose phosphate pathway in red blood cells or glycogen storage/glycogenolysis in liver or muscle) you should know it. You will NOT have to draw the structures of metabolites, but you will need to know how many of these metabolites are involved in regulating flux between various pathways. 9 . You will be given a copy of the basic metabolic map we have been referring to throughout the metabolism section (with thanks to Dr. Miesfeld). We hope this helps you answer the questions by and make connections without getting lost in the pathways. While studying, be sure to understand the different roles/fates of the key metabolites: acetyl CoA, glucose 6-phosphate, and oxaloacetate. 10. You still need to know the chemical properties of the amino acid side chains. They are like the alphabet of biochemistry! 11. Remember these equations; they will not be given on the exam: Change in Reduction Potential: E'= E' e acceptor - E' e donor Relating G' to E': G'= -nFE' The next page is the cover page of the exam so you know what to expect! WRITE YOUR NAME LEGIBLY ON EVERY PAGE CHECK TO BE SURE YOU HAVE 5 PAGES INCLUDING COVER PAGE. I swear/affirm that I have neither given nor received any assistance with this exam. Signature: Date: BIOCHEMISTRY 460 EXAM 3A April 28, 2010 Read each question carefully Budget your time wisely BE SURE TO STATE UNITS OF ANY NUMERICAL ANSWERS. USEFUL CONSTANTS: F (Faraday's constant) 96,500 JV1mol1= 96.5 kJV1mol1 ...
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- Spring '07