glycolysis

glycolysis - Bioc 460 Spring 2010 Lectures 23 and...

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Bioc 460 Spring 2010 Lectures 23 and 24:Glycolysis Dr. Lisa Rezende What is the purpose of glycolysis? How is it regulated? What happens when glycolysis and/or fermentation is disrupted? Image credit Morguefile http://mrg.bz/gByK6o
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Glycolysis learning objectives Explain the rationale behind the sequence of the steps in glycolysis in terms of free energy changes and ease for catalysis. Describe the net energy output of glycolysis and the free energy characteristics of the steps that drive the pathway in a forward direction. Identify the key regulatory steps in glycolysis, the enzymes and molecules involved regulating glycolysis, and explain how regulation occurs. Compare and contrast hexokinase to glucokinase. How do the biochemical properties of these two enzymes relate to their biological function? Describe the relationship between fermentation and maintenance of glycolytic flux in both yeast and mammals. Explain how the biological effects of mutations in genes encoding enzymes involved in glycolysis and fermentation. How does the biochemical defect lead to disease? Reading: Chapter 16 Problems: 3, 4, 8, 9, 10, 15, and 20 plus glycolysis problem set
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Triglyceride Synthesis (Glycerol moiety) Nucleotide biosynthesis, RNA, DNA Amino Acids, Protein (through TCA cycle) Glucose plays a central role in the metabolism of carbohydrates Adapted from Dr. T.S. Tsao ATP and carbon dioxide (Citrate cycle, ETS, Oxidative Phosphorylation)
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For the next two lectures, we will focus on the breakdown of glucose that prepares it for oxidation
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Image Credit Dr. R.L. Miesfeld
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Some key features of glycolysis -Occurs in the cytoplasm -Occurs under both aerobic and anaerobic conditions -Breaks down glucose from one 6 carbon glucose molecule to 2 3 carbon pyruvate molecules - Net synthesis of 2 molecules of ATP per glucose molecule -Reduces two molecules of NAD+ to NADH
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Net Reaction: Glucose + 2P i + 2 ADP + NAD + -> 2 pyruvate + 2 ATP + 2 NADH + 2H + + H 2 O When we are through, you should be able to explain where in the pathway each reactant in this diagram enters glycolysis and when each product is released.
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glycolysis - Bioc 460 Spring 2010 Lectures 23 and...

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