Bioc 460 Spring 2010 Quiz 6 Key ProteinMolecular Weight (kD)pIα-antitrypsin45.05.4Cytochrome c13.410.6Insulin5.85.4Src kinase60.04.81. (3 pts) Given the data in the table above, which two proteins would you expect to migrate to the samepoint on an isoelectric focusing gel? Why? α-antitrypsin and insulin (1 pt), because the pIs are the same (2 pt) 2. (3 Pts) Given the data in the table above, which protein would have a net positive chargeat physiological pH? Why? Cytochrome c (1 pt) because it’s pI > physiological pH (2 pt).
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