orgolab6.26.3

orgolab6.26.3 - Staci Williams Column Chromatography and...

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Staci Williams October 27, 2009 Column Chromatography and Thin Layer Chromatography Sections 6.2 and 6.3 Summary of Procedure: Perform column chromatography to separate fluorene and 9-flurenone from a 1:1 mixture, and then peform thin layer chromatogprahy to see how well the separation occurred. Procedure/Observations: Add sand to the chromatography column to form a layer about ½ inch thick, then add about 25mL of the elutant, which is the mobile phase using petroleum ether. Then slowly add 5g of dry neutral alumina, which is the stationary phase, forming a white column on top of the sand. Wash sides of column with petroleum ether, and then add another layer of sand atop the alumina. Drain column until the solvent reaches the top of the alumina. Add the misture to be separated by pipet, and then drain the sample into the sand layer. Rinse sides with elutant and then drain this rinse into the top sand layer. Add enough pure elutant to make the buret at least half full. Open stopcock and collect the elutant in an Erlenmeyer flask, testing solution on
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This note was uploaded on 05/08/2010 for the course PHYS v12 taught by Professor Adler during the Spring '10 term at NYU.

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orgolab6.26.3 - Staci Williams Column Chromatography and...

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