MCB 121 Lecture 15

MCB 121 Lecture 15 - MCB 121 - Lecture 15 MT 2 mean = 102...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
MCB 121 - Lecture 15 MT 2 mean = 102 +/- 20.3 High = 145, Low = 56 I. Alternative splicing. A) Type B) Mechanism II. RNAi introduction A) discovery B) Mechanism ----------------------------------------------------------------------------------------------- Fidelity of spliceosome: 1) recruitment of spliceosome in close proximity with the intron that's coming out 2) sequential processing A gene with 2 introns and 3 exons => Results two different proteins that come out from the same gene. They are protein isoferm, coming out the same gene but having different structure. Number of introns has a positive correlation between the complexity of the organism Signal cell eukaryote: has about 1 intron or 2 introns Higher eukaryote: worms and flies - 5~6 introns/gene, plant/mammal - 7 introns/gene on average. Number of intron per gene does not predict the number of protein isoforms come out due to alternative splicing. DSCAM-an extreme case of alternative splicing the protein in drosophila: roles in cell: 1) involves neural patterning 2) innate immune response exon 4 has 12 different 5' splice sites can be used Exon 4, 6, 9 and 17 each have alternative forms: 4 (x12), 6 (x48), 9 (x33) and 17 (x2). If each form can be spliced then the combination of forms equals 12 x 48 x 33 x 17= ~38,000 possible isoforms. There are several ways a message might be spliced. Here are some examples: One gene > multiple products
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
(account for different complexity of organisms even though there are different number of genes) => vast complexity due to pattern of alternative splicing. * intron retained (intron might contain coding region) There are two basic forms of alternative splice site regulation. 1. Splice site repressor 2. Splice site enhancer CASE: Sex determination of Drosophila based on splicing. - determine by the product of alternaitve splicing
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/09/2010 for the course MCB 121 taught by Professor Gasser during the Winter '09 term at UC Davis.

Page1 / 8

MCB 121 Lecture 15 - MCB 121 - Lecture 15 MT 2 mean = 102...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online