4 - 3A-throw it in a microscope direct detection Edited by...

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Principles of Diagnostic Microbiology Medical Microbiology Laboratory • Prior to the 1930’s medical microbiology laboratories were rare in North America and most diagnosis was based on clinical evidence alone • A massive review of medical schools lead to a complete reorganization of medical schools along the “John Hopkins Model”– Science finally arrives in medicine! • Laboratory work is needed for: – An accurate diagnosis – Determining which treatments will be effective – Providing a “Global Approach” to health care via Infection Control Medical Microbiology Laboratory A. Diagnosis - Direct Detection - Culture - Serology - Molecular B. Antibiotic Susceptibility - Specific isolate - Epidemiology - Treatment recommendations C. Infection Control - Screening - Organism typing - Isolation Procedures Diagnosis i) Direct detection - Microscopic • Common stains - Gram stain, Acid-fast stain, Giemsa stain, Others • Fluorescent dyes - Auramine rhodamine, Calcofluor white, Acridine orange • Darkfield - (eg. spirochetes) • Direct immunofluorescence - monoclonal or polyclonal antibodies Diagnosis i) Direct detection - Pathogen-specific macromolecules • Enzyme Immunoassay (EIA) eg. Streptococcal pharyngitis • Agglutination tests eg. Bacterial meningitis, organism typing
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4 - 3A-throw it in a microscope direct detection Edited by...

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