Diagnostic VirologyDiagnostic VirologyWhy bother? It is important information for:A.Transplantation↑viral infectionsHIV↑severity of infectionsB.Effective chemotherapy (the choice of anti-viral may depend on the nature of the virus)C.Epidemiology (we need to know what’s out there)Diagnostic VirologyRapid Dx:•↓tests / investigations•allow discontinuation of antibiotics•earlier effective treatment•early detection and management of epidemics•safe transplantation / transfusionDiagnostic VirologyThree common methods:1.Virus isolation2.Direct detection3. Serology4.PCR is comingDiagnostic Virology1.Virus Isolation:Common methods:• tissue culture• chick embryo• animal inoculationSpecimen Collection for Viral Culture•obtain material from infected organ(s)(not always possible or practical)•sampling of multiple sites (eg. resp. tract and urine for measles virus)•definitive diagnosis vs presumptive diagnosis(eg. measles virus vs enteroviruses)
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