1. There are seven different IS1 in Escherichia coli str. K-12 substr. MG1655 genome. They are very similar to each other, ranging from 90% to 100% identical. The differences first appear as mismatched base pairs spaced randomly throughout the sequence. As the differences mount, the first mismatches remain, but more mismatches are added as well. For example, in the most similar IS1, nucleotide changes appear at positions 50 and 262 (amongst others.) In the more different IS1, nucleotide changes appear at more positions, but the base changes at 50 and 262 remain. 2. When looking at Escherichia coli str. K-12 substr. DH10B genome, there are eleven different IS1. The distribution of IS1 elements is fairly similar in this very closely related strain. Although there are a few more elements, the elements present are also very similar and range from 90% to 100% identical. The genotype differences appear to be comparable too. The base pair changes between elements are spaced randomly. The changes also appear to occur at the
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