lecture0217audio - information about myoglobin: Publication...

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Alternatives to cDNA libraries  1) Purify the protein and sequence  it using mass spectroscopy 2) Sequence the entire genome of  an organism. Use rules and known  sequences to locate exons (protein  coding regions). Splice together  exons to create cDNA sequences.  Deposit sequences in a database.  Compare predicted cDNA with known  protein sequences in the databases to  identify proteins, chart the evolution of  the proteins Express cDNA sequences, localize  corresponding proteins, mRNA  etc.
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Accessing information about proteins on the  Web Information about protein  sequences, structures and scientific  papers can be accessed at the  National Center for Biotechnology  Information: www.ncbi.nlm.nih.gov
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Protein databases  At the NCBI website, you can use a  keyword (e.g. myoglobin) to access 
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Unformatted text preview: information about myoglobin: Publication abstracts (PubMed) (can get full papers from many journals if accessing through .umd) cDNA (nucleotide) sequence (mRNA sequence) Primary sequence of protein translated mRNA Structure Protein databases- BLAST searches At the NCBI website, you can also compare one sequence with others in the database. Can be used to classify unknown cDNA sequence that you have obtained from a tissue Can be used to search genomes and deposited cDNAs for homologous sequences create families and evolutionary lines Can be used to look for conserved motifs in known sequences. Can compare protein to protein sequences or translated cDNA to protein sequences...
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lecture0217audio - information about myoglobin: Publication...

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