App labophot2a

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Unformatted text preview: BASIC OPERATION OF THE NIKON LABOPHOT 2A MICROSCOPE This document describes the basic operating procedure for the Labophot microscopes when using them to examine opaque specimens. It starts by describing the procedure for bright-field reflected light microscopy and then it gives the basic procedures for polarized, dark-field and DIC imaging. Refer to figures 1, 2 and 3 if you have any difficulty finding any of the controls on this microscope. Tables 1 and 2 at the end of this document summarize that major specifications of these microscopes and figures 4 through 7 show examples of different imaging modes. Note: These microscopes are configured for reflected light microscopy. The objective lenses are all bright field/dark field, plano, achromat lenses and they do not include corrections for cover slips. Four of the objective lenses on one microscope also include DIC capabilities. Important: The objective lenses on these microscopes are all dry lenses, even the 100X objective. Using any immersion fluid can permanently damage them. Precautions 1. Handle the microscope gently. Strong mechanical shocks can damage it. 2. When carrying the microscope support it by the bottom of the base. 3. Do not use the full power settings for the illuminators. This will significantly reduce the life of the bulbs. 4. Be very careful to never let the specimen touch the objective lens. This microscope has features which will help you avoid this. Use them. 5. Never use oil or any other immersion medium with this microscope. All objective lenses are “dry” lens...
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This note was uploaded on 05/14/2010 for the course EMS 162l taught by Professor Staff during the Spring '08 term at UC Davis.

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