App-Labophot2A - BASIC OPERATION OF THE NIKON LABOPHOT 2A...

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Department of Chemical Engineering and Materials Science Mike Meier University of California, Davis September 13, 2004 B ASIC O PERATION OF THE N IKON L ABOPHOT 2A M ICROSCOPE This document describes the basic operating procedure for the Labophot microscopes when using them to examine opaque specimens. It starts by describing the procedure for bright-field reflected light microscopy and then it gives the basic procedures for polarized, dark-field and DIC imaging. Refer to figures 1, 2 and 3 if you have any difficulty finding any of the controls on this microscope. Tables 1 and 2 at the end of this document summarize that major specifications of these microscopes and figures 4 through 7 show examples of different imaging modes. Note: These microscopes are configured for reflected light microscopy. The objective lenses are all bright field/dark field, plano, achromat lenses and they do not include corrections for cover slips. Four of the objective lenses on one microscope also include DIC capabilities. Precautions 1. Handle the microscope gently. Strong mechanical shocks can damage it. 2. When carrying the microscope support it by the bottom of the base. 3. Do not use the full power settings for the illuminators. This will significantly reduce the life of the bulbs. 4. Be very careful to never let the specimen touch the objective lens. This microscope has features which will help you avoid this. Use them. 5. Never use oil or any other immersion medium with this microscope. All objective lenses are “dry” lenses. 6. Never force any part. Doing so might permanently damage the microscope. If something is stuck, then it should be serviced. In other cases there might be another way to make the needed adjustment. For example, do not twist the camera to line up a feature on the specimen with the edge of the field of view. Rotate the stage instead. Prepare the Microscope 7. Remove the cover and put it somewhere safe. 8. Inspect the microscope. 9. Turn on the epi-illuminator. Set the power to about 50%. Important: The objective lenses on these microscopes are all dry lenses, even the 100X objective. Using any immersion fluid can permanently damage them.
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Figure 1. A view of the right side of the Labophot microscope. (From Nikon’s instructions for the Labophot-2A.) Prepare the Specimen
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