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Unformatted text preview: ope. (From Nikon’s instructions for the Labophot-2A.) Initial Focus This simple step includes a very important safety procedure. 16. Slowly lower the specimen until it comes into focus. 17. Set the refocusing stopper clamp so that you won’t be able to crash the specimen into the objective lens. 18. Loosen the stage clamp screw and rotate the stage to orient the specimen to your liking. Tighten the screw again when done. Note: the polarizer, analyzer and the Wolfstram prism, if present, may need to be removed or adjusted so that you can obtain a clear, bright image of your specimen. Adjust Binocular Eye Pieces Properly adjusted eye pieces are essential for working comfortably with the microscope and for obtaining the best image in both eyes. 19. Set the distance between the eye pieces so that you can comfortably view the specimen by Figure 3. Identification of each part of the epi-illuminator. (From Nikon’s instructions for the Labophot-2A.) looking straight ahead using both eyes at the same time. 20. Close your left eye and focus the specimen using the fine focus knob. 21. Close your right eye and focus the specimen by turning the eye piece focusing ring. Adjust the Illumination A properly adjusted illumination system is necessary if you are to obtain the best resolution and contrast. 22. 23. 24. 25. 26. 27. Open the field and aperture diaphragms. Remove an eye piece. Look into the eye piece’s opening and observe the illumination of the specimen. Stop down the field diaphragm until it blocks about 20% of the view of the specimen. Replace...
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This note was uploaded on 05/14/2010 for the course EMS 162l taught by Professor Staff during the Spring '08 term at UC Davis.
- Spring '08