In reflected light microscopy the image is formed

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Unformatted text preview: of light. In reflected light microscopy the image is formed from light that is reflected from the edges of each feature, excluding light that is normally reflected from the flat areas. 32. 33. 34. 35. 36. 37. Set the illuminator’s power to maximum (on the last green LED, not the red LED). Open the aperture and field diaphragms. Remove the analyzer. Pull the BF/DF knob out to obtain dark field illumination. Focus. Adjust the polarizer to brighten the image. Note: Smooth specimens might not be good subjects for dark field imaging. Differential Interference Contrast (available on only one microscope) Differential interference contrast (DIC) can greatly enhance the quality of the image, bringing out the finer details in the specimen. It can also add striking but false colors and an illusion of depth. 38. Focus in the usual manner. 39. Make sure the analyzer is in. 40. Switch the Wolfstram prism to the “In” position. 41. Adjust the polarizer to obtain the desired effect. Note: Only the objective lenses marked “DIC” are capable of differential interference contrast imaging. Pulnix Video Camera Preparing the Pulnix video camera involves simply turning it on and performing a white balance. Some of our cameras have a white balance button you can press. Most do not. 42. Start up Adobe Photoshop and select file/import/plug-in digitizer. 43. Remove the specimen from the microscope. 44. Turn on the illuminator for transmitted light microscopy. Set it to 50% power. 44. Pull the knob to divert the light away from the eye pieces and to the camera. 45. Tur...
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This note was uploaded on 05/14/2010 for the course EMS 162l taught by Professor Staff during the Spring '08 term at UC Davis.

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