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Unformatted text preview: es. 6. Never force any part. Doing so might permanently damage the microscope. If something is stuck, then it should be serviced. In other cases there might be another way to make the needed adjustment. For example, do not twist the camera to line up a feature on the specimen with the edge of the field of view. Rotate the stage instead. Prepare the Microscope 7. Remove the cover and put it somewhere safe. 8. Inspect the microscope. 9. Turn on the epi-illuminator. Set the power to about 50%. Department of Chemical Engineering and Materials Science University of California, Davis Mike Meier September 13, 2004 Figure 1. A view of the right side of the Labophot microscope. (From Nikon’s instructions for the Labophot-2A.) Prepare the Specimen Care must be taken to prepare a specimen whose reflecting surface is perpendicular to the optic axis and to install it in the microscope without damaging the specimen or the microscope. 10. 11. 12. 13. 14. 15. Mount the specimen on microscope slide. Use some modeling clay, lens paper and the Leitz press. Press only hard enough to get the specimen to stick to the clay and the square the specimen with the optic axis. Release the refocusing stopper clamp. Lower the specimen stage. Select the 5X objective. This is the shortest objective and it has the longest working distance and the largest depth of field. Install the specimen. Raise specimen slide to within 2-4 mm of the objective lens, less than the working distance and yet a safe distance from the lens. Figure 2. A view of the left side of the Labophot microsc...
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This note was uploaded on 05/14/2010 for the course EMS 162l taught by Professor Staff during the Spring '08 term at UC Davis.
- Spring '08