Biochem Chapter 15

Biochem Chapter 15 - 1) Arsenate can act as a substrate for...

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1) Arsenate can act as a substrate for any reaction in which phosphate is a substrate. Write a balance overall reaction for the conversion of glucose to pyruvate in presence of ATP, ADP, NAD+ and either A) phosphate Glucose + 2NAD+ +2ADP + 2Pi 2 Pyruvate + 2NADH+ 2 ATP + 2H2O. B) Arsenate. Glucose+ 2NAD+ +2ADP+ 2AsO4(3-) 2 Pyruvate+2NADH+ADP-AsO3(2-) + 2H2O 2ADP-AsO3(2-)+2H2O 2ADP + 2AsO4(3-) Overall: Gluose + 2NAD+ 2 pyruvate + 2NADH. C ) Why is arsenate a poison? Because it uncouples ATP generation from glycolysis. Consequently, glycolytic energy generation cannot occur. 2) Although it is not a primary flux- control point for glycolysis, pyruvate kinase is subject to allosteric regulation. A) what is the metabolic importance of regulating flux through the pyruvate kinase reaction? Pyruvate kinase regulation is important for controlling the flux of metabolites, such as fructose in liver, which enters glycolysis after the PFK step. B)
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Biochem Chapter 15 - 1) Arsenate can act as a substrate for...

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