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Biochem Chapter 16

Biochem Chapter 16 - 1 Write the balanced equation for a...

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1) Write the balanced equation for a ) the sequential conversion of glucose of pyruvate and of pyruvate to glucose . Overall reaction for glycolysis is Glucose + 2 NAD+ + 2Pi 2 Pyruvate + 2NADH+ 4H+ + 2ATP + 2H2O. Overall reaction for gluconeogenesis is 2Pyruvate + 2NADH + 4H+ +4ATP + 2GTP + 6H2O Glucose + 2NAD+ + 4ADP + 2GDP + 6Pi For the two processes operating sequentially, 2ATP + 2 GTP +4H2O 2ADP + 2 GDP + 4Pi B) The catabolism of 6 molecules of G6P by pentose phosphate. The Reaction for catabolism of 6 G6P by the pentose phosphate is 6 G6P + 12 NADP+ + 6H2O 6 Ru5P + 12NADPH + 12H+ + 6H2O 6Ru5P + H2O 5 G6P + Pi The Net equation is G6P + 12 NADP+ + 7H2O 12NADPH + 12H+ +6CO2 + Pi. 2) Phosphoglucokinase catalyzes the phosphorylation of the C6-OH group of G1P. Why is this enzyme important for the normal function of phosphoroglucomutase? Phosphoglucokinase activity generates G1,6P which is necessary to “prime” phosphoglucomutase that has become dephosporylated and thereby inactivated through the loss of its G1, 6P reaction intermediate.
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