BIO 325 - Class notes - 100609 and 100809

BIO 325 - Class notes - 100609 and 100809 - EEEM IO/G jo‘...

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Unformatted text preview: EEEM IO/G jo‘? FIOKBVVU‘CC ~— CHA ’Q‘B'JYCKC AYOVVKDSLOMOK/e [Giacg 02L DIV/Ar WM axe/3’11“}; CLEVELfia/xormous/Y 0-247 flu (Ll/UVOWxO o‘v’n—k v" C.) VCUZCW W ll MW =m Clown/7 W Hawk f’M/Uvi 0L1? m .5 &V\‘J'\\)3€O'RC Mlklfilmtfl jag/1AM _ wgixhtxnm 473 Nam/7 [email protected] M (M41 M C COW VCCC’LKVUL Magmx‘C/QA) ,_ OWL“) rd; +0 zoo'g of K); 1w K}; ,5 #MWQ: ’“ ’"1’ COM? /CQH 1+“wa PL $ "A a F '1‘ 4\ 1\ ‘ tibmw Wdtifmxf fiiOfldg (MA/r é off H of a” f) RLC/C \‘kafi ( % J W CNme/vxoxom‘ ; \ A \ 1 a7 \ f ( \ f \ S '5 ”x Q \N. ,. .../ /\ TQCincA/ut’ WW kw MCQJ‘W(X olOWC/P’DNA) *“ RON} ~ m‘vda mM GT DNA \ (NF (1‘ 1&4fo lo)8)o‘l HF ___, . z” . €614 hmfir‘hufl‘ (1,0,4 SAKS 7K as‘is '7LOM9 lac”3G/a“‘§iy __f____,____ a. DNA NON/MSW ()kyi‘r/LS M q_ 7%qu '50!“ CA W (ELM/kw COM/W03 WVLQ I WW4 W Ovi {W (o) . ’0‘ Info/Wis iV\1L€?"VQ%OW shied "it F ext/wt cWUWoSC/WLQ» Clvcuzcmij ‘ {y W WMMS ‘1 1 Q g 4‘ flflflflfl ,_ I . fi 3 0 FW 1ch PW 77M, +7W \7/‘2’ 99/43ng ‘ ' “’ffit/V “- ’Y‘QIOVILLOM of ESQWW "k; (“W Qon is €‘fi’3'CkiMA {A}. {QQJ/L Ma!) {‘8 W h f’iVSTL flAOk/fl‘lCCl'HQ/L Magi fiqcmd CLWUMOScwj m C/QVCUZa/v {3+ "— cfla+ avfl'l' («pf Sex/f W+W+ 55/5 C Mm VHNCd/L m fa/loufiry HILVSJVCLW'L; VW Cit/vivvtcij . “— W —— fl ._ __ ,— M. _ 7‘ #5:... m m1: W jofi—W—fi-W limefifm mfmualajcu’r w—Lwcbx. mAmcuvka/t/ iii: CLPELCLVS in 111 YflCi‘IENt/U" aid? (flak MEL" 50W" "MA/'7” M4 7‘ "— 2Q I 2. -— - A; 7’ C? Q: -~ — 3 _ Z ’ " ,7 I I -— 29 i) Ow <1 Sig/4, chd‘u/cuv mafi/ Show W o'rfgl‘m Quack OWNLVLCM 01L ”)YCLV‘tgzLLy of "max/kw} f-m/ 41mph Hf“ show/x. 1i) Skew W. OUSJ—nmu At-JVLLLOAA m wwvlcamr'n 'vm‘nul—Q/J GENETICS [BIO 325! In a cross of an Hfr strain that is met‘i3 erg": 5110’“, his", str'5 with an F' strain that is met‘, arg', are", his', strr, recombinants are selected that that can grow on a minimal medium supplemented with different amino acids. What is the donor marker that is being selected for in the recombinants on medium (a) without methionine (met) but with arginine (arg), aromatic amino acids (are), and histidine (his) and in the presence of streptomycin (str)? (‘0) without arginine (arg) but with methionine (met), aromatic amino acids (arc), and histidine (his) and in the presence of streptomycin (sir)? (c) without histidine (his) but with methionine (met), arginine (arg), and aromatic amino acids (are), and in the presence of streptomycin (sir)? Four Hfr strains of E. 0011' are known to transfer their genetic material during conjugation in different sequences. Given the time of entry of the markers into the F‘ recipient, construct a genetic map which includes all of these markers and label the distance (in minutes) between adjacent genes. Strain l markers arg — thy — met — thr time (min) 15 21 32 48 Strain 2 markers nial — met — thi — thr 4 try timc(min) 10 17 22 33 5? Strain 3 markers phe — his — bio — azi — thr — thi time (min) 6 ll 33 48 49 60 Strain 4 markers his — phe — arg — mal time (min) 18 23 35 45 Show the order of transfer of genes to an F' cell from Hfr strains obtained by crossing—over of the F plasmid with the donor chromosome as shown in the. following examples. The arrowhead is the first segment of the F plasmid that enters the recipient cell. (a) (b) (C) M‘kflgirfl ‘7’ achwafl CLWQMOSOI/NM ‘” 4W “ of ’ “”7 WM”? *fl‘ Wii‘fix‘fiifi ”f ‘F‘ZN‘SCcl/Qfl. MOE/3F”? __ QLQA&5L on WCOME}r\qW{— /7 WWW/key (MM + 7413 7% Md“ + $4“) ,_______:—————-—"’ W Sewl‘eC!’ LQJJL+ %comLinamJfi ‘4 WP“ {flaxmf‘fii Ta” 00’3”? 3 M4 (Umsdakofl map/MM) + ~ * °’ _..._ www rim-“:7 M “4 kiwi/‘7 *‘ Rafi" OWE," W4 (HQ/UK } ’V‘W(_ — (3/ng ._..-_——. ime wxofeculcv/ wcombivmkm ( \L 150% «mun/1W \ (T‘QCOW ~Q‘6Q0VS} Uaam/fi floi- £1LQ\S\?V\ O/V‘Q Cl‘L - F WWflACK‘T' mac owthwflfc‘fl “1—1th {73wa (WHO V\ w. mgbquvCSI/vx 0+ fro/wt Nexus/4W ) Pmbqb/Y {VON/{A fo/ {*QKCWV—fi Of (‘2ch I‘m/flVWCL‘l‘l‘Cf/L I‘vx {bath/Km! bail—{CL +0 5L I‘hvo/mot 1‘“ fiWVFGUv ‘* ”“0”?“ “W hmjslam for MA wimpy Oman gum/Y MUM/4m for ECLCMA "'— NCHUYCLI 'NQRSPWLMHM abomcl f“ 0* 7LUAJ EQCMQ , {bam'llvs 305%”; can "17*“ ufs my _ Swisbcoccus iYWLMOI/VCM bNA COM/x Jun u!) ‘ Neisseyfia m~enfniflt§om «- ID A?! UfU‘aLg S{,ovu,y\w DNA WH’PL Ufy/Tkkk SQ; (M -v~ Coal/amg-jbwwxcmtfovx MHOW/Kfjj (‘3 DNA {amok/C £011,777 of“ L‘Ow C r}, )E/ 0: Jr ’3 71 0‘ +WM—T+ M (—‘J 20 " L‘ ‘0 f 9 0w / M . Io "9 C "" 0‘ Jr ,__ MLMMWAW F‘" 3‘ M 01+ E 3 — H) r ...
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