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BIS 104 prot - liposome spring 2010

BIS 104 prot - liposome spring 2010 - A liposome...

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Analyze membrane Protein Function by Isolation and Reconstitution 1. Solubilize membrane proteins by treating isolated plasma membrane with non- ionic detergent (e.g., Triton X-100 or octylglucoside) 2. Separate protein of interest from lipids and other proteins. 3. Remove remaining detergent. 4. Reassemble proteins of interest in a lipid bi-layer of known lipid composition (liposome).
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Unformatted text preview: A liposome preparation can be controlled in the following ways: Lipid composition (relative proportion of PS, PC, cholesterol, etc) Contents of the lipid vesicle Diameter of the vesicle Protein content of the bi-layer Liposome surface properties Antibody allows targeting to specific tissues Polyethylene glycol (PEG) creates “stealth” liposomes...
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