finalkey - 1 BIS 101 - 003 Final March 21, 2009 READ THE...

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1 BIS 101 - 003 Final March 21, 2009 READ THE FOLLOWING INSTRUCTIONS: Write your name legibly on each page of this exam. All work must be done in ink . Show all your work. Points will be subtracted for each incorrect answer in multiple-choice questions. However, you will not receive a negative total score for such a question. This exam is worth 200 points. You have 110 min. to complete the exam. Good Luck!
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2 Name:______________________________ ID _________________________ Page: 3 ____14____ 4 ____14____ 5 ____18____ 6 ____22____ 7 ____14____ 8 _____8___ 9 ____12____ 10 ____18____ 11 ____14____ 12_____16___ 13 ____16___ 14 _____24___ 15 _____10___ Total ________
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3 Name:_________________________ (1) Loci A and B are linked on the chromosome. The genetic distance between the loci is 10%. If the dihybrid AB / ab is testcrossed, what genotypes and in what proportions do you expect? (6 points) Gametes Gametes 0.45 AB 0.05 Ab 0.05 aB 0.45 ab ab 0.45 AaBb 0.05 Aabb 0.05 aaBb 0.45 aabb (2) Most of the erminette fowl (erminette is the name of the breed of chickens) are light colored with occasional black one, giving a flecked appearance. A cross of two erminettes produced a total of 48 progeny, consisting of 24 erminettes, 14 blacks and 14 pure white. (a) What genetic basis of the erminette pattern do you suggest? (4 points) The segregation seems to fit a 1 black : 2 erminette : 1 white ratio indicationg segregation of a single incompletely dominant gene. (b) Test statistically your hypothesis using χ 2 (4 points) Observed Expected Balck 14 13 1/13 0.077 Erminette 24 26 4/26 0.153 White 14 13 1/13 0.077 52 52 0.308 0.7 < P (2 d.f.) < 0.9. Accept the hypothesis Ho that the observed ratio fits the 1 : 2 : 1 ratio.
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4 Name:_________________________ ( 1 ) Below is a diagram of a eukaryotic gene, its primary RNA transcript, mRNA and the polypeptide it encodes. Do the following. (14 points) (a) Label the 5' and 3' ends on all DNA and RNA strands. (b) Indicate which DNA strand is a template and which is a nontemplate. Draw in a TATA box where it should be. (c) Show where the transcript starts on the primary (nascent) transcript (shorten or elongate the line if needed). (d) Show where the mature mRNA starts and where its ends (shorten or elongate the line if needed) and draw in all modifications that distinguish it from the primary transcript. (e) In the polypeptide, indicate where it starts and where it ends relative to the landmarks on the DNA molecule (shorten or elongate the line if needed), and label the amino- and the carboxy- termini. D N A 5’ __________+1_____ATG______________________TAA___________ 5’ 3’ ___________________________________________________________ 3’ primary RNA transcript +1 AUG UAA 5’ ____________________________________________ 3’ mRNA +1 AUG UAA 5’ Cap ________________________________________AAAAAAAAAA 3’ polypeptide N terminus Met___________________________ C terminus
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Name:________________________
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finalkey - 1 BIS 101 - 003 Final March 21, 2009 READ THE...

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