Unformatted text preview: size alpha globin protein, not associated with the microsomes. Therefore, a single protein band on SDS gel, that would be digestible by protease. (2) absence of microsomes: Same as above; only native alpha globin protein is made. (In describing your results remember that the protein products from the cell-free system are analyzed by SDS-PAGE). c) Why is the procedure described in (b) above an important control for the in vitro experiments described by Lingappa? It demonstrates that native alpha globin protein, in the absence of a signal sequence, does not associate non-specifically with an in vitro preparation of microsomes....
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- Spring '09
- alpha globin protein, Lingappa