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Unformatted text preview: facilitate fusion? Additional SNAREs; fusogenic proteins; Ca ++ ions. c. What additional proteins are involved in vesicle fusions in vivo? Rabs and tethering proteins. d. What techniques would you use to actually visualize or measure whether or not fusion between liposomes had occurred? The two liposome preps could be made to include two different fluorochromes in their lumen. If fusion occurred, the fluorochromes would intermix and the resulting new color could be seen by fluorescence microscopy. Alternatively, the fluorchromes could be attached to lipids that make up the liposomal membranes. If liposomes with two different fluorochromes fuse, lateral movement resulting in intermixing of the markers could be seen by fluorescence microscopy. (like the Frye and Edidin experiment)...
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- Spring '09