bis_104_pq_27_ans_09

bis_104_pq_27_ans_09 - BIS 104 PQ 27 ans 09 You are...

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BIS 104 PQ 27 ans 09 You are interested in studying the role of lipid composition in SNARE-mediated fusions. Using the in vitro fusion assay, another member of your lab previously observed that addition of the cone-shaped phosphatidic acid (PA), to 85 mol percent phosphatidylcholine (PC):15 mol percent phosphatidylserine (PS) acceptor liposomes at the expense of PS increases the rate of fusion; addition of inverted-cone shaped lyso-phosphatidic acid (LPA) was inhibitory. You are interested in testing the ability of two lipids found in biological membranes, diacylglycerol (DAG) and phosphatidylinositol (PI), to either stimulate or inhibit fusion. 1) Given your previous findings, predict what the effects would be of including in acceptor liposomes either PI or DAG on SNARE-mediated fusion rates. DAG, like PA, is a cone-shaped lipid, with a relatively small headgroup (a hydroxyl ion); the prediction would be that addition of DAG to proteoliposomes in the assay would increase the rates of fusion, as cone-shaped lipids tend to induce the membrane curvature required for
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bis_104_pq_27_ans_09 - BIS 104 PQ 27 ans 09 You are...

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