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Unformatted text preview: (1) presence of microsomes: Since the mRNA has no signal sequence code, the translation system would synthesize native size alpha globin protein, not associated with the microsomes. Therefore, a single protein band on SDS gel, that would be digestible by protease. (2) absence of microsomes: Same as above; only native alpha globin protein is made. c. Why is the procedure described in (b) above an important control for the in vitro experiments described by Lingappa? It demonstates that native alpha globin protein, in the absence of a signal sequence, does not associate non-specifically with an in vitro preparation of microsomes....
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- Spring '09