Restriction Endonuclease Analysis of Lambda DNA using NEB cutter.docx

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Restriction Endonuclease Analysis of Lambda DNA using NEB cutter Nivisan Asokan 500837019 BLG-888 Sec 11 Martina Hausner Venezia Benvenuto March 4 , 2020
Results Table 1. Fragment sizes of Lambda DNA of Single Restriction Enzyme Digestion Using NEBcutter V2.0 a single digestion of the lambda DNA using Eco RI, Bam HI, and Hin DIII was done. A 0.7% agarose gel was used and the fragment lengths were given. Eco RI Bam HI Hin DIII 21226 16841 23130 7421 7233 9416 5804 6770 6682 5643 6527 4361 4878 5626 2322 3530 5505 2027 564 1
Table 2. Fragment sizes of Lambda DNA of Double Restriction Enzyme Digestion Using NEBcutter V2.0 a double digestion of the lambda DNA using Eco RI + Bam HI, and Eco RI + Hin DIII was done. A 0.7% agarose gel was used and the fragment lengths were given. Eco RI + Bam HI Eco RI + Hin DIII 15721 21226 5505 5148 4669 4973 3775 4268 3758 3530 3530 2027 3240 1904 2752 1709 2564 1375 1868 947 1120 831 564 2
Table 3. Restriction Site and Fragment Number Generated from Each Digestion The number of restriction sites and number of fragments generated from each digestion was quantified in order to reveal the relationship between the two. The NEBcutter V2.0 software was used in order to perform these digestions. Number of Restriction Sites Number of Fragments Eco RI 5 6 Bam HI 5 6 Hin DIII 6 7 Eco RI + Bam HI 10 11 Eco RI + Hin DIII 11 12 3
Figure 1. Eco RI and Bam HI Single and Double Digestion Restriction Map of Lambda DNA A restriction map was created for the following digestions; single digestion using Eco RI (A), a double digestion using Eco RI and Bam HI (B), and a single digestion using Bam HI (C). The restriction sites for each enzyme is seen in each set of digestions of a 48502bp Lambda DNA. E represents the restriction sites for Eco RI and Bam HI is shown a B. The lengths of each fragment is also shown in base pairs.

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