100%(5)5 out of 5 people found this document helpful
This preview shows page 1 - 6 out of 10 pages.
Restriction Endonuclease Analysis of Lambda DNA using NEB cutterNivisan Asokan500837019BLG-888 Sec 11Martina Hausner Venezia BenvenutoMarch 4, 2020
ResultsTable 1. Fragment sizes of Lambda DNA of Single Restriction Enzyme DigestionUsing NEBcutter V2.0 a single digestion of the lambda DNA using EcoRI,BamHI, andHinDIII was done. A 0.7% agarose gel was used and the fragment lengths were given.EcoRI BamHIHinDIII2122616841231307421723394165804677066825643652743614878562623223530550520275641
Table 2. Fragment sizes of Lambda DNA of Double Restriction Enzyme DigestionUsing NEBcutter V2.0 a double digestion of the lambda DNA using EcoRI + BamHI, andEcoRI+ HinDIII was done. A 0.7% agarose gel was used and the fragment lengths were given.EcoRI + BamHIEcoRI + HinDIII15721212265505514846694973377542683758353035302027324019042752170925641375186894711208315642
Table 3. Restriction Site and Fragment Number Generated from Each DigestionThe number of restriction sites and number of fragments generated from each digestion was quantified in order to reveal the relationship between the two. The NEBcutter V2.0 software was used in order to perform these digestions. Number of Restriction SitesNumber of FragmentsEcoRI 56BamHI56HinDIII67EcoRI + BamHI1011EcoRI + HinDIII11123
Figure 1. EcoRI and BamHI Single and Double Digestion Restriction Map of Lambda DNAA restriction map was created for the following digestions; single digestion using EcoRI (A), a double digestion using EcoRI and BamHI (B), and a single digestion using BamHI (C). The restriction sites for each enzyme is seen in each set of digestions of a 48502bp Lambda DNA. E represents the restriction sites for EcoRI and BamHI is shown a B. The lengths of each fragment is also shown in base pairs.