Lec 6 - Lec 6 Use of Microscopes Limits of Resolution...

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Lec 6 Use of Microscopes Limits of Resolution Unaided human eye >200um Light microscope <200 nm (1000 fold magnification) Electron microscope: (5000-6000 fold magnification) Theoretical 0.002 nm Achievable 0.100 nm Average Cell diameter 20-20 um Bacterium 1-2 um Ribosome 0.02 um
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Biomembrane 0.006 um (lipid bilayer + protein) (wavelength is limiting factor of microscope) The Light Microscope (Chapter 18.1-18.2) -Light source (photons) at the bottom. -Wavelength of visible light range from Violet deep red about 400nm to 750 nm (white light) -wavelength below 400 nm is the ultra violet light, longer than 750 nm will be infrared -lens collection system that captures all light that diffuse out of light source and focus on plane of system (condenser lens)- capture as much as light is possible and focus plane of light on specimen examined -the medium is between the cover slip and the objective lens (water, oil, air) -objective lens is most expensive part, give magnifying power, quality of image you will see, has to be designed for medium
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