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Lec 7 - Lec 7 Microscopy magnification based on the product...

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Lec 7 Microscopy – magnification based on the product of magnification you achieve by the objective lens x ocular eye piece lens = total magnification for that microscope 1. Light –bright field –phase contrast + IDC –fluorescence 2. Electron microscopy (Em) – lambda is not the wavelength of visible light photons but the lambda is the wavelength of high energy electrons. This number becomes really small. Resolving power of Em is 2 or 3 magnitude greater than that of a light microscopy –TEM –SEM (resolution is ability of microscope to view two separate points in a specimen, 2 organelles or 2 small objects on your microscope and be able to distinctly resolve them into 2 entities, so you can distinctly see 2 objects into 2 entities) d= [(0.6)(lambda)]/[n*sin (alpha)] = [(0.61)(lambda)]/NA Objective lens over slip specimen stage field diaphragm control light (400- 800nm) Empty Magnification- what happens when you take a situation like this and increase the magnifying power. Will introduce a higher lens or increment of magnification, but it does not increase detail, it will just make it bigger. Will still appear to you as a large object then a single object. Gone beyond the resolution power of the microscope, resolution is the limiting factor. Resolution limiting factor is the quality of the lens and how efficient is that objective lens at capturing the light from the lens (sin alpha) and function of wavelength of light -want to make d as small as possible.
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