Lec 8 - Lec 8 Microscopy Fluorescence-scanning...

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Lec 8 Microscopy Fluorescence -scanning confocal -deconvolution Electron microscopy (Em) -TEM; SEM Biomembranes LIGHT MICROSCOPY I. Bright field microscopy Magnification Resolution Contrast Modify the specimen: specific dyes (color) for cell components Modify the microscope: II. Phase-contrast microscopy Visualize internal components of living cells (unstained) at relatively high resolution III. Fluorescence microscopy Fluorochromes- absorb and excited by photons of particular wavelength and responds by giving off lower energy at higher wavelength Epi-fluorescence Scanning confocal fluorescent microscopy Deconvolution fluorescent microscopy Other applications: Photobleaching – involves chemical phenomenon where fluorochrome is activated but fluorochrome will actually go out later Fluorescence recovery after photobelaching (FRAP) – use fluorochrome and fluorescence, how close two molecules are or two different part f single molecule are. Fluorescence resonance energy transfer (FRET) Fluorescence-activated cell sorter (FACS) – physically separate out 2 or more population of cell from a mixed population of cells. Separate 2 homogenous cell type
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Lec 8 - Lec 8 Microscopy Fluorescence-scanning...

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