Lec 8 - Lec 8 Microscopy Fluorescence -scanning confocal...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Lec 8 Microscopy Fluorescence -scanning confocal -deconvolution Electron microscopy (Em) -TEM; SEM Biomembranes LIGHT MICROSCOPY I. Bright field microscopy Magnification Resolution Contrast Modify the specimen: specific dyes (color) for cell components Modify the microscope: II. Phase-contrast microscopy Visualize internal components of living cells (unstained) at relatively high resolution III. Fluorescence microscopy Fluorochromes- absorb and excited by photons of particular wavelength and responds by giving off lower energy at higher wavelength Epi-fluorescence Scanning confocal fluorescent microscopy Deconvolution fluorescent microscopy Other applications: Photobleaching – involves chemical phenomenon where fluorochrome is activated but fluorochrome will actually go out later Fluorescence recovery after photobelaching (FRAP) – use fluorochrome and fluorescence, how close two molecules are or two different part f single molecule are. Fluorescence resonance energy transfer (FRET) Fluorescence-activated cell sorter (FACS) – physically separate out 2 or more population of cell from a mixed population of cells. Separate 2 homogenous cell type
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

Lec 8 - Lec 8 Microscopy Fluorescence -scanning confocal...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online