Lec 9 - Lec 9 Light Microscope The resolution and...

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Lec 9 Light Microscope The resolution and magnification by light microscopy is limited by constraints. Make d as small as possible (resolving power of the microscope). Violet (400nm). Useful is about 1000-1500 fold magnification. You won’t get increase of clarity of image or resolution even if you use different ocular piece (empty magnification). Because of wavelength constraint the lambda value can’t go lower than 400 nm and the resolving power and effective magnification that can be achieved my light microscopy is limited by 1000 fold diameter. Use visible light photons as illumination/visualizing medium. Focusing power is glass lenses. -Can observe live tissue/cell without causing much damage for a long period of time. Electron Microscopy Reduce the wavelength/resolution several magnitude smaller from 400 nm. Use high energy electron generated in electron microscope. 10000 smaller and higher energy than light photons. -can only view dead material, tissue must be fixed and stained. (very critical, to not
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This note was uploaded on 05/19/2010 for the course BIS BIS 104 taught by Professor Privalsky during the Spring '09 term at UC Davis.

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Lec 9 - Lec 9 Light Microscope The resolution and...

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