Notes 10-20-08

Notes 10-20-08 - Recombinant DNA consists of two pieces of...

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Recombinant DNA consists of two pieces of DNA ligated together into a single molecule -can occur in vivo (in the cell) and in the test tube also plasmids can replicate themselves independently of the host chromosome (use the host’s replication machinery; have a specific origin of replication); often referred to as cloning vectors PRC used to amplify a piece of DNA of interest 3 steps Denaturing-heat the DNA to separate DNA into two strands [breaks the hydrogen bonds between the base pair nucleotides] Annealing-cooling (first add synthetic primers (with 3’ hydroxyls), add dNTPS (A, G, C, T), add DNA polymerase which recognizes the primers Extension-DNA polymerase adds nucleotides to the 3’ ends of the primers Total DNA molecule produced = 2 n copies n =number of copies ______________________________________________________________________ signal transduction pathway (for cellular communication) -signal reception (mediated by a receptor protein positioned on the cell membrane) activated receptor passes on communication to
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This note was uploaded on 05/20/2010 for the course BIOL 101 taught by Professor Fevzidaldal during the Fall '08 term at UPenn.

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Notes 10-20-08 - Recombinant DNA consists of two pieces of...

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