6. You are studying the transport activity of Na+K+ATPase in sealed “right side out”erythrocyte ghosts. It is possible to adjust the contents of the inside of the ghostsduring their preparation. Beside each substance below writeINSIDEorOUTSIDEto designate whether it must be present inside or outside the ghost to maintainenzyme activity.a) Na+______________b) ATP ______________c) Ouabain inhibits the activity of Na+K+ATPase by binding to the K+ bindingsite of this enzyme. Must ouabain be on the inside of the ghost or the outside of theghost to inhibit the Na+K+ATPase activity?7. Beside each portion of the cell listed below, write either “inside” or “outside” todesignate whether it is topologically related to the inside or the outside of the cell.Interior of lysosome ____________________Nuclear matrix ____________________Cytosol ____________________Lumen of endoplasmic reticulum ____________________Space between the two mitochondrial membranes ____________________8. Midtermase is a soluble enzyme that can be isolated from the lysosomes of fibroblasts.
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Ouabain, Midtermase, Na K ATPase, translat ion/translocat ion