Bis 104 etzler Q - Page 3

Bis 104 etzler q page 3

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Unformatted text preview: washed and then SDS was added. The differences between the samples are summarized in the table below. Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 No microsomes Microsomes Microsomes Microsomes Microsomes Protease added after SDS Deglycosylat ing enzyme added after SDS Protease added and then remo ved by washing befo re adding SDS 9. A rapidly growing yeast culture was pulsed for two minutes with 3 H­leucine and then washed and chased with medium containing unlabeled leucine. At various time intervals after the beginning of the chase, aliquots were removed from this culture and fract ionated as fo llows: (A) The cells were separated from the cell medium by low speed centrifugat ion. (B) The cell pellet fro m step (A) was washed wit h buffer and then disrupted by sonication in the presence of sucrose. (C) The homogenate from step (B) was sub...
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This note was uploaded on 05/24/2010 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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