washed and then SDS was added. The differences between the samples aresummarized in the table below.Tube 1No microsomesTube 2MicrosomesProtease added after SDSTube 3MicrosomesDeglycosylating enzyme added after SDSTube 4MicrosomesTube 5MicrosomesProtease added and then removed by washing before adding SDSThe contents of each of the tubes were then run in SDS gel electrophoresis, followedby exposure to film to detect the labeled protein. Each lane on the film(autoradiogram) below corresponds to one of the above samples (but they are notnecessarily in order). In the circle above each lane, place the number of the abovetube containing the sample that should give the results shown in that lane. (Note:the direction of electrophoretic migration is from top to bottom ... i.e. the smallestproteins are at the bottom of the gel. You can assume that the molecular weight of a
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