This preview shows page 1. Sign up to view the full content.
Unformatted text preview: jected to preparative ultracentrifugat ion in dilute sucrose at a speed and time sufficient to pellet all o f the organelles. De mo The contents of each of the tubes were then run in SDS gel electrophoresis, fo llowed by exposure to film to detect the labeled protein. Each lane on the film (autoradiogram) below corresponds to one of the above samples (but they are not necessarily in order). In the circle above each lane, place the number of the above tube containing the sample that should give the results shown in that lane. (Note: the direct ion of electrophoretic migrat ion is fro m top to bottom ... i.e. the smallest proteins are at the bottom of the gel. You can assume that the mo lecular weight of a signal pept ide is 3,000 and the molecular weight of either type of carbohydrate unit is 3,000.)...
View Full Document
This note was uploaded on 05/24/2010 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.
- Spring '08