bis_104_final_exam_key_fall_2008 - Page 2

bis_104_final_exam_key_fall_2008 - Page 2 - a protein...

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8. a reporter gene _______________________ is often incorporated into DNA constructs in order to determine if and when a specific gene has been expressed at the protein level (translation has occurred). 9. SDS-PAGE ________________________ is a technique that uses an electric field to separate proteins based solely on their molecular mass. 10. western blot _______________________ is the technique used to probe for and detect proteins that have been resolved by the technique described in #9 above. 11. cell-free translation system ____________is an in vitro procedure that can be used to synthesize
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Unformatted text preview: a protein encoded by a specific mRNA. 12. fluoresence activated cell sorting (FACS) _ is a fluorochrome-laser based system that can be used to separate out specific cell types from a mixed population of live cells. Page 2 Name ____________________________________ PART II: Identification (24 pts) For each of the items listed below, describe its i) location and composition, and ii) its function(s) A. HMG-CoA reductase i) Enzyme (protein) located in the SER of animal cells. ii) HMG-CoA reductase catalyzes the rate limiting step in the cholesterol biosynthetic pathway . Demo...
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